Journal: Neuropharmacology

Article Title: AMP-activated protein kinase slows D2 dopamine autoreceptor desensitization in substantia nigra neurons

doi: 10.1016/j.neuropharm.2019.107705

Figure Lengend Snippet: Summary of compounds used. Concentrations (Conc) refer to final concentrations in slice superfusate or pipette solutions, whereas Solvent refers to stock solutions.

Article Snippet: A769662, ZLN024, STO609 acetate, PKA Inhibitor fragment(6-22) amide (PKI), forskolin and (−)-quinpirole hydrochloride were purchased from Cayman Chemical (USA).

Techniques: Transferring, Solvent, Diffusion-based Assay

Journal: Neuropharmacology

Article Title: AMP-activated protein kinase slows D2 dopamine autoreceptor desensitization in substantia nigra neurons

doi: 10.1016/j.neuropharm.2019.107705

Figure Lengend Snippet: A) Both A769662 and ZLN024 significantly slowed the rundown of dopamine-induced current compared to control. The combination of A769662 plus ZLN024 evoked no outward current in the absence of dopamine. B) A769662 and ZLN024 increased sulpiride-sensitive currents recorded in the last 5 min of dopamine superfusion. C) Bath application of AMPK inhibitors dorsomorphin (30 μM) and STO609 (10 μM) reversed the slowing of current rundown that is produced when pipettes contained A769662 (10 μM). The combination of dorsomorphin and STO609 evoked no outward current in the absence of dopamine D) Dorsomorphin and STO609 blocked the ability of A769662 to increase sulpiride-sensitive currents. Current-decay plots were analyzed with a mixed model followed by Sidak pairwise comparison tests, whereas sulpiride-sensitive currents were analyzed with Welch’s t tests: **, P < 0.01; ***, P < 0.001.

Article Snippet: A769662, ZLN024, STO609 acetate, PKA Inhibitor fragment(6-22) amide (PKI), forskolin and (−)-quinpirole hydrochloride were purchased from Cayman Chemical (USA).

Techniques: Control, Produced, Comparison